Parallel RNAi screens reveal cell line-specific phenotypes. (a) Different cell lines exhibited different hit rates in RNAi screens (Additional file 3). (b) Venn diagrams depict the segregation of screen hits between related cell lines. (c) A Venn diagram depicts the classification of hits into three distinct classes: those that are hits in both CNS and hemocyte cell lines; those that are hits in neuronal cell lines only; and those that are hits in hemocyte cell lines only. (d) Hierarchical clustering of hits across cell lines (depicted in the form of a tree) was used to give a more detailed picture of the three hit classes. Two hits of particular interest, CG7236 and minibrain (mnb), are highlighted. Note that the relationships defined by the functional analysis (depicted in the form of a tree at the top of figure) mirror the relationships defined by the microarray analysis (see Table 1 for the Pearson correlation coefficients in each case).