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Figure 3 | Genome Biology

Figure 3

From: The origin recognition complex protein family

Figure 3

ORC and its interactions with other pre-RC proteins at origins of DNA replication. Orc1-Orc5 are required for origin recognition and binding in S. cerevisiae, whereas Orc6 is dispensable in this regard [44]. In contrast, Orc6 is essential for ORC DNA binding in D. melanogaster [28]. Studies with both S. cerevisiae and human cells have indicated that Cdc6 interacts with ORC through the Orc1 subunit (indicated by a double arrow) [31, 79, 80]. This association increases the specificity of the ORC-origin interaction [20]. Further studies with S. cerevisiae suggest that hydrolysis of Cdc6-bound ATP promotes the association of Cdt1 with origins through an interaction with Orc6 (indicated by a double arrow) [25, 31], and this in turn promotes the loading of Mcm2-7 helicase onto chromatin.

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