| N. furzeri | N. kunthae | ||
---|---|---|---|---|
 | GRZ: Sanger sequencing* | GRZ: pyrosequencing†| MZM-0403: Sanger sequencing* | Sanger sequencing* |
Number of sequence contigs | 5,540 | 1,095,308 | 5,686 | 6,273 |
Average length ± SD (bp) | 968 ± 446 | 102 ± 15 | 948 ± 408 | 855 ± 432 |
Range of length (bp) | 101-2,685 | 36-230 | 100-2,699 | 100-2,738 |
Total sequence (bp) | 5,364,828 | 111,563,506 | 5,364,828 | 5,366,245 |
Number of uncalled bases | 6,661 | 32,643 | 3,809 | 3,786 |
Percentage of uncalled bases | 0.12 | 0.03 | 0.07 | 0.07 |
Percentage of bases with Phred‡ ≥40 | 84.1 | 1.3 | 75.1 | 76.1 |
Percentage of bases with Phred ≥30 | 89.5 | 22.5 | 83.6 | 85.0 |
Percentage of bases with Phred ≥20 | 93.2 | 89.2 | 89.7 | 91.2 |
G+C content (%) | 44.9 | 44.3 | 44.3 | 44.9 |