High tandem repeat content in the genome of the short-lived annual fish Nothobranchius furzeri: a new vertebrate model for aging research

Table 1 Whole genome shotgun sequences

	N. furzeri			N. kunthae
	GRZ: Sanger sequencing*	GRZ: pyrosequencing^†	MZM-0403: Sanger sequencing*	Sanger sequencing*
Number of sequence contigs	5,540	1,095,308	5,686	6,273
Average length ± SD (bp)	968 ± 446	102 ± 15	948 ± 408	855 ± 432
Range of length (bp)	101-2,685	36-230	100-2,699	100-2,738
Total sequence (bp)	5,364,828	111,563,506	5,364,828	5,366,245
Number of uncalled bases	6,661	32,643	3,809	3,786
Percentage of uncalled bases	0.12	0.03	0.07	0.07
Percentage of bases with Phred^‡ ≥40	84.1	1.3	75.1	76.1
Percentage of bases with Phred ≥30	89.5	22.5	83.6	85.0
Percentage of bases with Phred ≥20	93.2	89.2	89.7	91.2
G+C content (%)	44.9	44.3	44.3	44.9

*Sanger sequencing was performed on ABI 3730xl machines. ^†Pyrosequencing was performed on Roche/454 GS20 sequencers; sequences were not assembled. ^‡Phred ≥40 corresponds to at least 99.99% accuracy; Phred ≥30 to 99.9% accuracy; Phred ≥20 to 99% accuracy. SD, standard deviation.

ISSN: 1474-760X