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Table 1 Whole genome shotgun sequences

From: High tandem repeat content in the genome of the short-lived annual fish Nothobranchius furzeri: a new vertebrate model for aging research

  N. furzeri N. kunthae
  GRZ: Sanger sequencing* GRZ: pyrosequencing MZM-0403: Sanger sequencing* Sanger sequencing*
Number of sequence contigs 5,540 1,095,308 5,686 6,273
Average length ± SD (bp) 968 ± 446 102 ± 15 948 ± 408 855 ± 432
Range of length (bp) 101-2,685 36-230 100-2,699 100-2,738
Total sequence (bp) 5,364,828 111,563,506 5,364,828 5,366,245
Number of uncalled bases 6,661 32,643 3,809 3,786
Percentage of uncalled bases 0.12 0.03 0.07 0.07
Percentage of bases with Phred ≥40 84.1 1.3 75.1 76.1
Percentage of bases with Phred ≥30 89.5 22.5 83.6 85.0
Percentage of bases with Phred ≥20 93.2 89.2 89.7 91.2
G+C content (%) 44.9 44.3 44.3 44.9
  1. *Sanger sequencing was performed on ABI 3730xl machines. Pyrosequencing was performed on Roche/454 GS20 sequencers; sequences were not assembled. Phred ≥40 corresponds to at least 99.99% accuracy; Phred ≥30 to 99.9% accuracy; Phred ≥20 to 99% accuracy. SD, standard deviation.