Identification of novel transcripts with differential dorso-ventral expression in Xenopus gastrula using serial analysis of gene expression

Table 3 Set of selected tags, tag-mapping and experimental validation

ID	Matches to genome	Bioinformatics mapping	rSAGE mapping	X. laevis homologue	Validation
DV01	23	pintallavis	pintallavis	pintallavis	Positive control
DV02	14	3 EST clusters	-	ND	ND
DV03	10	vent1.1	-	vent-1.1	Positive control
DV04	26	No transcript	Scaffold_19023: 2428-2444	Not found	In situ
DV05	1,482	6 transcripts	Cluster Str. 39849	Not found	PCR and in situ
DV06	2	goosecoid	goosecoid	goosecoid	Positive control
DV07	1	zcsl-2	zcsl-2	LOC496356	False positive
DV08	82	LOC496648/ubadc1	ubadc1	MGC115132	False positive
DV09	20	sox11	sox11	sox11	In situ
DV10	7	admp	admp	admp	Positive control
DV11	3	LOC100124861	-	MGC82245	False positive
DV12	9	LOC549498	LOC549498	MGC115377	In situ
DV13	12	Id2	id2	id2	PCR and in situ
DV14	10	No transcript	Cluster Str.3968	MGC82755	False positive
DV15	11	vent1.2	-	vent1.2	Positive control
DV16	3	MGC147163	MGC147163	MGC81848	PCR and in situ
DV17	11	Str.45862/Str.40022	Str.45862/Str.40022	Not found	PCR
DV18	68	LOC548724	LOC548724	MGC116509	In situ
DV22	9	smagp	ND	mitotic phosphoprotein 22	PCR and in situ
DV25	27	cyclin A2	ND	MGC130969	False positive
DV38	25	nap1l1	ND	nap1	PCR and in situ

Summary of the tag-mapping and experimental validation of selected tags. Dashes (-) indicate rSAGE failed to provide a longer and specific sequence. ND, not determined.

ISSN: 1474-760X