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Table 1 Description of dorsal and ventral SAGE libraries

From: Identification of novel transcripts with differential dorso-ventral expression in Xenopus gastrula using serial analysis of gene expression

SAGE library

Dorsal

Ventral

Total

Sequenced colonies

1,265

1,018

2,283

Repeated ditags

2,183 (12.2%)

359 (2.2%)

2,542 (7.4%)

Ditags*

15,773

16,057

31,830

Tags

31,538

32,104

63,642

Linker tags

363

57

420

Total experimental tags

31,175

32,047

63,222

Unique experimental tags

14,546

14,486

23,766

Experimental tags matching to the genome

14,352

14,347

23,455

  1. SAGE libraries were prepared from total RNA of dorsal and ventral explants of X. tropicalis gastrula. Concatemer sequences were processed for tag extraction and comparison between libraries. *Repeated ditags were considered only once. Tags including 'N' in the sequence were not considered (eight tags in the dorsal library and ten tags in the ventral library).