Microsynteny between homologous miRNA clusters conserved between A. thaliana, rice (O. sativa) and poplar (P. trichocarpa). VISTA plots [37, 70] shows the conservation of different clustered miRNAs in the three selected genomes (Table 1; Additional data file 2): (a-d) the four rice miR395 clusters; (e) the Ath-MIR166c, d cluster; (f) the Ath-MIR169i-n cluster. To analyze evolutionary conservation between monocots, the sorghum genome is indicated. In each graph, gene models (blue for protein coding gene exons, and red for miRNA clusters) are indicated above, and percentage (50 to 100%) on the right side indicates the level of identity between target and reference genomes, visualized through pink and blue peaks for intronic/non-protein coding, and exonic regions, respectively. The name of syntenic clusters in non-reference genomes is indicated, and syntenic clusters are delimited by black bars. Ath, Arabidopsis thaliana; Ptc, Populus trichocarpa; Osa, Oryza sativa, Sbi, Sorghum bicolor.