Subject enrolment and study design. (a) Recruitment strategy. A total of 598 subjects consisting of 29 uninfected controls and 569 patients diagnosed with sepsis were recruited in this study. Of the patients diagnosed with sepsis (569 subjects), only those with positive blood cultures (63 subjects) were included for further study. Subjects who had no signs of infection (29 subjects) were also recruited to constitute an uninfected control group, including healthy donors, patients diagnosed with T2D, and patients who had recovered from melioidosis. Subjects for this latter group could not be recruited in our second validation set. (b) Study design. The diagram depicts the composition of the training and independent test sets. Of 92 subjects enrolled in this study, 34 were assigned to the training set, 33 were assigned to the test set 1, and 25 were assigned to the test set 2. T2D, type 2 diabetes.