Figure 1From: Genome-wide analysis reveals rapid and dynamic changes in miRNA and siRNA sequence and expression during ovule and fiber development in allotetraploid cotton (Gossypium hirsutum L.)Sequencing flow chart and size distribution of small RNAs in cotton. (a) Flow chat of small RNA library construction and sequencing. The plant materials included seedling leaves, dissected ovules 3 days prior to anthesis (-3 DPA), on the day of anthesis (0 DPA), and 3 days post-anthesis (+3 DPA). Red arrows indicate the location of materials harvested for RNA extraction. (b) Size distribution of small RNAs in leaves and ovules at -3 DPA, 0 DPA, and +3 DPA. High-level accumulation of 24-nucleotide small RNAs in the ovules (0 and +3 DPA) may result from overproduction of siRNAs during early stages of ovule and fiber development. Inset: total small RNA reads after removal of other cellular RNA sequences.Back to article page