Figure 2

ESE and ESS gains and losses after gene duplication. (a) Overall losses of ESEs are observed in different types of paralogous exon pairs (AC, AA, CC) when compared to the genome-wide difference (Orth group). Futhermore, the AC and AA groups are significantly different from the CC group. Changes of control motifs are also plotted to see how splicing affects the evolution of ESEs. (b) Unlike the ESEs, no significant differences were found when compared to genomic differences (the Orth group). Similarly, the control motifs are displayed beside the ESS motifs. (c) The relative difference of ESEs is negatively correlated with Ka, and (d) no correlation is found for ESSs. A corresponding plot with Ks as time proxy is shown in Additional data file 2. Error bars in (a, c) show standard errors in each group.