Subnuclear localization of the duplicated var2csa genes in HB3CSA parasites. Representative DNA FISH pictures illustrating the five different localization patterns of both var2csa paralogs (green), telomeric clusters (rep20, red) in nuclei stained with DAPI (blue). I, co-localized var2csa alleles where both also co-localized with rep20; II, co-localized var2csa alleles that did not co-localize with rep20; III, non-co-localized var2csa alleles but both co-localized with rep20; IV, non-co-localized var2csa alleles with neither allele co-localized with rep20; V, non co-localized var2csa alleles with one allele co-localized with rep20. The var2csa paralogs were exclusively found towards the rim of the nuclei. (b) Quantification of the described localization patterns illustrated in a pie-chart with percentages (n = 204). Co-localized var2csa paralogs (I and II) are shaded with stripes and constitute 78.5% of the total.