Targeted next-generation sequencing of a cancer transcriptome enhances detection of sequence variants and novel fusion transcripts

Table 1 Analysis of Illumina sequence in cDNA hybrid selection

Sequence filter criteria	Before^a	After^a
Total purity-filtered reads	7,907,124	7,635,761
Aligned to all transcriptome	4,515,009	6,664,152
Unique in transcriptome	4,303,769	6,508,099
Mapping to 1 of 887 target transcripts	220,151	6,364,131
On-target specificity	5%	98%

ISSN: 1474-760X