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Table 5 Assembly statistics

From: Deep short-read sequencing of chromosome 17 from the mouse strains A/J and CAST/Ei identifies significant germline variation and candidate genes that regulate liver triglyceride levels

Assembler Sequencing Strains Insert size Read length (bp) Number of reads Raw read coverage Assembled bases (Mb) Contig coverage Contig N50
FuzzyPath Illumina CAST/Ei 200 bp 36 173 million 65× 76.03 80.0% 2,315
Velvet* Illumina CAST/Ei 200 bp 36 173 million 65× 58.09 61.15% 391
ABySS Illumina CAST/Ei 200 bp 36 173 million 65× 72.80 76.63% 1,022
FuzzyPath Illumina A/J 200 bp 36 112 million 42× 55.87 58.8% 959
Phusion Capillary A/J 3-5 kb 400-900 357,100 2.5× 81.2 85.5% 3,377
FuzzyPath Hybrid A/J Hybrid Hybrid Hybrid Hybrid 85.54 90.0% 5,793
  1. *Version 0.4. We also tried the latest version 0.7, which had been much improved in terms of contig length, but ran out of memory on a computer with 192 Gb RAM. Assembly incorporating the A/J Illumina reads and Celera capillary reads.