Skip to main content

Table 6 RT-qPCR validation of SAGE profile for rostral E15.5 and caudal E15.5 regions

From: Molecular networks involved in mouse cerebral corticogenesis and spatio-temporal regulation of Sox4 and Sox11 novel antisense transcripts revealed by transcriptome profiling

   

Fold change

SAGE tag

RefSeq accession

Gene ID

Caudal/rostral (SAGE)

Caudal/rostral (RT-qPCR)

gttgttcttccagtcgg

NM_016916

bladder cancer associated protein homolog (human), Blcap

2.68

1.43

gtcatagctgttctgtg

BC025816

EST sequence BC025816

Cs

1.31 (NS)

aagcttgacatttggaa

NM_026187

ankyrin repeat and zinc finger domain containing 1, Ankzf1

Rs

1.17† (NS)

gatacttggaatgacta

NM_007393

actin, beta, cytoplasmic, Actb

-2.63

-1.32

ttggtgaaggaaaaaac

NM_021278

thymosin, beta 4, X chromosome, Tmsb4x

-2.33

-2.13 (NS)

  1. All RT-qPCR data are statistically significant at P < 0.05 unless specified. †A disagreement between RT-qPCR and SAGE data. Cs: caudal region-specific expression; NS: no statistically significant difference between two developmental stages; Rs: rostral region-specific expression. Fold change values of <1.0 are presented in a negative fold change format.