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Table 6 RT-qPCR validation of SAGE profile for rostral E15.5 and caudal E15.5 regions

From: Molecular networks involved in mouse cerebral corticogenesis and spatio-temporal regulation of Sox4 and Sox11 novel antisense transcripts revealed by transcriptome profiling

    Fold change
SAGE tag RefSeq accession Gene ID Caudal/rostral (SAGE) Caudal/rostral (RT-qPCR)
gttgttcttccagtcgg NM_016916 bladder cancer associated protein homolog (human), Blcap 2.68 1.43
gtcatagctgttctgtg BC025816 EST sequence BC025816 Cs 1.31 (NS)
aagcttgacatttggaa NM_026187 ankyrin repeat and zinc finger domain containing 1, Ankzf1 Rs 1.17 (NS)
gatacttggaatgacta NM_007393 actin, beta, cytoplasmic, Actb -2.63 -1.32
ttggtgaaggaaaaaac NM_021278 thymosin, beta 4, X chromosome, Tmsb4x -2.33 -2.13 (NS)
  1. All RT-qPCR data are statistically significant at P < 0.05 unless specified. A disagreement between RT-qPCR and SAGE data. Cs: caudal region-specific expression; NS: no statistically significant difference between two developmental stages; Rs: rostral region-specific expression. Fold change values of <1.0 are presented in a negative fold change format.