In situ RNA hybridization of selected R versus C regions of E15.5 DETs. ISH was performed on (a-m) E15.5 and (n-t) E11.5 to P150 brains. (g-m) Coronal sections that are generated from the rostral to caudal axis; (a-f, s-t) sagittal sections. Micrographs of higher magnification are presented directly after any micrographs with an inset box (d-e, m, t). All micrographs are in dark-field except for (e, m), which are bright-field micrographs. C: caudal; CB: cerebellum; CP: cortical plate; DG: dentate gyrus; HPf: hippocampal formation; IZ: intermediate zone; OB: olfactory bulb; R: rostral; VZ: ventricular zone. Arrows show the region with expression or silver grains.