Target prediction of locust miRNAs. (a) Left side: distribution of target number of locust miRNAs. Right side: distribution of target site number of the unigenes. (b) Presumable pairing between lmi-miR-1 and LM00689 with highest score predicted by miRanda. (c) Conservation of mir-133 target site in the pale gene of locust (lmi) and 12 Drosophila species, and presumable pairing between miR-133 and the pale gene. The red boxes indicate conserved target sites of miR-133 in 3' UTR sequences of pale.