Figure 3
From: Characterization and comparative profiling of the small RNA transcriptomes in two phases of locust
Principles of locust-specific miRNA prediction and examples of the secondary structure of locust-specific miRNA precursors. (a) The features of miRNA and other small RNAs. Left side: the red and green lines represent the mature miRNA and miRNA*, respectively, which can be found in the same small RNA library sequenced by high-throughput sequencing in most cases. The black circles show the 1-2 nucleotide 3' overhang of miRNA:miRNA* duplex. Right side: inconsistency at the 5' ends of other small RNAs and the degradation fragments. (b) Electrophoretic analysis of PCR products of lmi-novel-04 and lmi-novel-07, showing the expected length of 55-70 nucleotides. (c) The secondary structures of lmi-novel-04 and lmi-novel-07. The red sequence represents mature miRNA and the green represents miRNA*. The black circles indicate 1-2 nucleotide 3' overhangs.