Conservation of miRNA* in the locust. (a) Electrophoretic analysis of PCR products amplified by the primer pairs designed on the basis of predicted miRNA* as based on a similarity to fruit fly miRNA* and their corresponding mature miRNAs. For each miRNA, the left lane is the negative control and the right lane is the positive result. (b) Two examples of precursor sequences of seven conserved miRNAs that have a conserved star sequence. The alignment of mir-276 and mir-307 in different insects shows high conservation of their miRNA*. The green nucleotides represent miRNA star sequence and the red represent mature miRNA sequence. The asterisks indicate the conserved sites among these species. (c) Hairpin structures of the mir-276 and mir-307 precursors of the locust. aga, A. gambiae; ame, A. mellifera; bmo, B. mori; dme, D. melanogaster; lmi, L. migratoria.