Figure 2

T-DNA integration patterns in pathogenicity mutants. (a) Representative transformant with a single T-DNA integration, resulting in one fragment with either restriction enzyme or probe (lanes 1 and 2). (b) Representative transformant with a double unlinked T-DNA integration, resulting in two fragments with either restriction enzyme or probe (lanes 1 and 2). (c) Representative transformant with a double inverted T-DNA integration fused at the LB, resulting in one fragment in the BglII digestion hybridized with the gpdA or trpC probe (BglII, lanes 1 and 2), one fragment of 8.4 kb in the BamHI digestion when hybridized with the gpdA probe (BamHI, lane 1) and two fragments when hybridized with the trpC probe (BamHI, lane 2). (d) Representative transformant with a double inverted T-DNA integration fused at the RB, resulting in one fragment in the BglII digestion when hybridized with the gpdA or trpC probe (BglII, lanes 1 and 2), a fragment of 2.2 kb in the BamHI digestion when hybridized with the trpC probe (BamHI, lane 2) and two fragments when hybridized with the gpdA probe (BamHI, lane 1). (e) Representative transformant with a single T-DNA integration and a second aborted T-DNA integration event. (f) Representative transformant with more than one T-DNA and with binary vector DNA. Blots were hybridized with gpdA (lane 1), trpC (lane 2), LB (lane 3), RB (lane 4) and pPZP (lane 5) probes. This figure is a composition of different blots, which results in minor differences in apparent fragment sizes. The negative results for the pPZP probe for the transformants depicted in (b-f) are omitted for clarity.