Figure 5
From: Studying alternative splicing regulatory networks through partial correlation analysis
Examples of EE links illustrated by RT-PCR of tissue RNAs. (a) Scheme of RT-PCR design to examine splicing levels of alternative exons. Primers (arrows) are in the flanking constitutive exons. Inclusion levels of alternative exons (black box) are calculated as Included form/(Included form + Skipped form). (b). Alternative splicing of Kifap3 exon20, St7 exon 7, and Map3k7 exon 12 in multiple mouse tissues. Kifap3 exon 20 is positively correlated with St7 exon 7 and negatively correlated with Map3k7 exon 12. St7 exon 7 is negatively correlated with Map3k7 exon 12. Pair-wise Pearson correlations based on the RT-PCR experiments are shown. (c) Slc35b3 exon 4 is positively correlated with Rai14 exon 11. (d) Pair-wise correlations between Kifap3 exon20, St7 exon 7, and Map3k7 exon 12 in a second set of tissues not surveyed by the Affymetrix exon array. (e) Pair-wise correlation between Slc35b3 exon 4 and Rai14 exon 11. Percentages of inclusion levels were averaged from three independent experiments.