Table 3 Deletion phenotypes predicted differently under raffinose conditions by iLL672 builds with and without α-galactosidase activity
Gene | Experimental phenotype of deletion under YPRaff | Predicted phenotype of deletion mutant made by iLL672 build with α-galctosidase | Predicted phenotype of deletion mutant made by iLL672 build without α-galctosidase | Reason for incorrect prediction made by iLL672 build with α-galactosidase activity |
|---|---|---|---|---|
Gal1 | Viable | Unviable | Viable | Galactose is produced when α-galactosidic bond in raffinose is cleaved. Therefore, in build with α-galactosidase, Gal genes are required to metabolize galactose produced during raffinose catabolism |
Gal7 | Viable | Unviable | Viable | See explanation for Gal1 |
Gal10 | Viable | Unviable | Viable | See explanation for Gal1 |
YBR184W | Viable | Unviable | Viable | YBR184W is the putative α-galactosidase and is essential by definition of the model build |
Suc2 | Unviable | Viable | Unviable | The galactose produced when the α-galactosidic bond is cleaved can be used as the primary carbon source, with the remaining sucrose unit being excreted |
Pgi1 | Unviable | Viable | Unviable | Pgi1 catalyzes the reversible conversion of glucose-6-phosphate to fructose-6-phosphate in glycolysis and gluconeogenesis. The pentose phosphate pathway can be used to bypass the deletion of this gene to maintain glycolytic function Without the α-galactosidase only the fructose unit of raffinose is usable, making Pgi1 essential for its role in gluconeogenesis |