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Figure 7 | Genome Biology

Figure 7

From: Model-driven analysis of experimentally determined growth phenotypes for 465 yeast gene deletion mutants under 16 different conditions

Figure 7

Fluxes through central energy metabolism in the iFF708 model under glycerol conditions. The VisANT network visualization software (see Materials and methods) was utilized to display flux distributions predicted by the iFF708 model as edges on a graph containing the reactions and metabolites participating in yeast central energy metabolism. For both networks, red nodes represent reactions and blue nodes represent metabolites. Edges between reactions and metabolites are indicative of metabolites being either reactants or products in the given reaction. The thickness of a particular edge is indicative of the relative flux through the reaction, where all fluxes are normalized by the maximal flux through an individual network. Predicted flux distributions are shown for (a) the wild type under glycerol conditions and (b) a mutant lacking complex III of the electron transport chain under glycerol conditions. Mutant flux distributions were computed here using the FBA assumption of optimal growth. The most prominent difference between the mutant and wild-type fluxes is the diversion of flux from the respiratory chain to the reaction in pyrimidine biosynthesis catalyzed by Ura1. This incorrect rerouting of flux in the respiratory mutant results in the incorrect prediction by the model that respiratory function is not essential when glycerol is the primary carbon source.

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