Figure 4
From: Gene expression regulation in the context of mouse interspecific mosaic genomes
Assessment of reproducibility of the microarray data. (a) Linear regression analysis for the 60 genes located in an approximately 10 Mb region of M. spretus origin (MMU19) shared by 44H and 137F strains, and of M. musculus origin in the B6 and 97C strains (Figures 2, 3 and 7). The x-axis represents the log2 of the expression ratios between 137F and B6. The y-axis represents the log2 of the expression ratio between either 44H and B6, or 97C and B6. As expected, there is a highly significant correlation between the 44H/B6 ratio versus the 137F/B6 ratio (blue dots), since 44H and 137F both contain a segment of M. spretus origin at this chromosomal location, while there is no significant correlation between the 97C/B6 ratio versus the 137F/B6 ratio (red dots), due to dysregulations described in the text. (b) Linear regression between expression levels obtained by microarray or quantitative RT-PCR (QRT-PCR) for a sample of twelve genes.