Figure 1
From: The miR-17-5p microRNA is a key regulator of the G1/S phase cell cycle transition
Phase enriched expression of the miR-17-92 locus. (a) HeLa cells were synchronized by double-thymidine block and synchrony was assessed by flow cytometry analysis of propidium iodide stained cells. DNA profiles are show from top to bottom as follows: asynchronous cells, S-phase cells (T = 0 h; synchrony >97%), G2/M phase cells (T = 8 h; synchrony >93%), and G1/GO phase cells (T = 14 h; synchrony >74%). Within each profile, cells classified as G1/G0 are depicted in dark blue, S-phase are depicted in orange, and G2/M are depicted in light blue. (b) Graph showing relative expression of miR17-92 pri-miRNAs in synchronized HeLa S phase, G2/M phase, and G1/G0 phase cell populations as assessed by qRT-PCR. (c) Graph showing relative expression of miR17-5p mature miRNAs in synchronized HeLa S phase, G2/M phase, and G1/G0 phase cell populations as assessed by qRT-PCR (mean ± SEM).