Genomic analysis of the relationship between gene expression variation and DNA polymorphism in Drosophila simulans

Table 2 Gene feature length, polymorphism and divergence in males for genes with high, average and low levels of expression

	Low	Average	High	Tukey's HSD summary*	X ²	p-value^†
Number of genes	2,073	4,167	1,709
Length
EXON	1,874	1,747	1,225	L>A>H	306.91	***
5'UTR	282	240	207	L>A>H	16.62	0.0002
Intron	3,644	2,521	1,450	L>A>H	7.11	0.0286
Number of introns	3.86	3.75	2.88	L=A>H	63.89	***
3'UTR	503	380	338	L>A>H	77.77	***
Polymorphism
CPR	0.0277	0.0295	0.0290	L=A=H	13.40	0.0012
5'UTR	0.0114	0.0116	0.0097	L=A>H	24.01	***
Nonsynonymous	0.0029	0.0023	0.0016	L>A>H	245.83	***
Synonymous	0.0335	0.0322	0.0277	L>A>H	86.68	***
First intron	0.0290	0.0276	0.0263	L≥A≥H	7.58	0.0226
All introns	0.0317	0.0301	0.0284	L≥A≥H	9.52	0.0086
3'UTR	0.0131	0.0122	0.0109	L=A>H	41.23	***
Divergence^‡
CPR	0.0493	0.0533	0.0528	A=H>L	19.77	***
5'UTR	0.0225	0.0232	0.0208	A≥L≥H	12.66	0.0018
Nonsynonymous	0.0066	0.0060	0.0047	L>A>H	155.62	***
Synonymous	0.0524	0.0543	0.0494	A≥L≥H	35.69	***
First intron	0.0475	0.0463	0.0433	L=A>H	7.79	0.0203
All introns	0.0483	0.0492	0.0462	A≥L≥H	7.06	0.0293
3'UTR	0.0237	0.0225	0.0204	L=A>H	22.83	***

*L, low expression; A, average expression; H, high expression (see Materials and methods). ^†X² and p-values derived from Kruskal Wallis; three asterisks denote p-value < 0.0001. ^‡Divergence refers to lineage specific divergence along the D. simulans branch.

ISSN: 1474-760X