Metabolic pathway coverage: glycolysis/gluconeogenesis. Component enzymes of the glycolysis/gluconeogenesis pathways predicted to be present in Toxoplasma from genome analysis are colored. Virtually every component of the glycolysis/gluconeogenesis pathway predicted for Toxoplasma was identified as being expressed in tachyzoites by proteomic analysis. Green and blue indicate genes for which expression has been confirmed in tachyzoites in this study by mass spectrometric data; blue also signifies genes for which post-translational modification is likely as indicated by the evidence from two-dimensional gels. Red indicates genes for which expression of predicted components has not been confirmed in this study. Coverage of key metabolic pathway component proteins was determined using the Metabolic Pathway Reconstruction for T. gondii available on the KEGG Pathway site accessed via ToxoDB .