Subcellular localisation and functional categorization of the expressed tachyzoite proteome. The numbers correspond to the total number of identified proteins in each category. (a) Protein subcellular localization information was first assigned according to gene descriptions and GO annotation provided by ToxoDB. When no information was available, protein sequences were submitted to PATS, PlasMit and WoLF PSORT. The combined results were manually assessed to obtain subcellular localization predictions. A detailed list of proteins in each subcellular localization to accompany this figure is provided in Additional data file 12. (b) Functional categorization was constructed using the GO classifications listed on ToxoDB for each release4 gene, which were then assigned to specific MIPS categories within the FunCatDB functional catalogue. Genes without a GO classification were assigned a putative MIPS category using additional information provided by Blast, Pfam domain alignments, InterPro and from independent literature searches. Notes: protein fate includes protein folding, modification and destination. A detailed list of proteins in each functional category to accompany this figure is provided in Additional data file 13.