Peptide evidence indicating alternative exon positioning and sequence annotation. The position of ORF X-1-3917326-3920484 in the genome scaffold is indicated by a red line on the grey track at the top of the figure and this region is expanded below, the red triangle demarking the ORF length. Predicted exons are indicated as blue boxes, linked by zigzag lines to indicate the position of exon/intron boundaries. Gene 28.m00300 is shown with two exons. ESTs are shown as dark blue or brown boxes. Peptides aligning with this region are shown in yellow. The predicted sequence for ORF X-1-3917326-3920484 is shown as an insert and sequence that matches exon 2 of gene 28.m00300 is shown in blue. Sequence for which there is matching peptide evidence is shown in red. Purple lettering indicates the positioning of the 'intron-located' peptide, mass spectrometric evidence for which is shown in the right hand insert.