Figure 4
MYLK isoform expression in ONH astrocytes. (a) Representative double immunofluorescent staining of MYLK (red) and astrocyte marker GFAP (green) in sections of human ONH from an AA donor (51 year old male), AAG donor (70 year old male), CA donor (56 year old female) and CAG donor (76 year old male). Nuclei (blue) are stained with DAPI. Note strong granular staining of MYLK in astrocytes (arrows) in the cribriform plates of the lamina cribrosa of AA and AAG donors compared to CA and CAG donors. V, blood vessel; NB, nerve bundle. Scale bar 35 μm. (b) Representative western blots of astrocyte cell lysates with MYLK antibody. β-Actin was used as a loading control. Note that AAG1-4 donors express more MYLK-210 and less MYLK-130 than CAG1-4 donors. (c) Graph of MYLK-210 expressed as the fraction of MYLK-210 in the four groups. (d) Graph of the fraction of MYLK-130 expressed in the four groups. These results represent densitometry analysis of western blots using seven AA, five AAG, eight CA and eight CAG donor samples.