SOTA clustering of temporal expression profiles from qRT-PCR analysis of 44 genes in pancreas development. qRT-PCR was used to determine the relative expression levels of the indicated genes during pancreas development at the TSs indicated. The relative level of expression of each gene was normalized and a SOTA analysis used to group the genes. Heatmaps of the relative expression levels of the genes in the SOTA groups, including the SOTA centroid, with peak expression in (a) the islets, (b) the TS26 developing pancreas, (c) the TS21-TS26 developing pancreas, or (d) the ducts are shown. The data shown are averages of the results obtained from pancreases from three separate litters (pancreases from an individual litter were pooled) or islet/duct collections with triplicate reactions from the separate RNA extractions.