Lentiviral RNAi library screen of human kinases identifies regulators of cancer cell growth. (a) Target genes covered by the shRNA library were classified according to gene function using Gene Ontology groups . (b) Two human sarcoma cell lines, HT1080 and U-2-OS cells, were infected with lentiviruses containing shRNAs targeting human kinases in 384-well format. Genes whose knockdown inhibits growth of either cell line by more than 25% compared to control with statistical significance (P < 0.01) were considered as hits. This diagram shows the number of hits specific to each cell line and common to both cell lines. (c) Median value of percent growth inhibition (%GI) from triplicate results in each cell line. The top seven hits were selected based on the summed value of %GI in both cell lines.