From: Assignment of isochores for all completely sequenced vertebrate genomes using a consensus
Method meets criteria | ||||||||
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Evaluation criteria | Experimental evidence | References | IsoF | GC-P | BASIO | L-S | Consensus | |
1. | Isochore border between the genes MN1 (in the GC rich region) and PITPNB (in the GC poor region) in the human genome | Replication time during the S phase of the cell cycle | [30] | Yes | Yes | Yes | No | Yes |
Early MN1 gene, late PITPNB gene | ||||||||
Pause of about 3 hours at isochore border | ||||||||
2. | Isochore border within the KIAA1043 gene in the human genome | Replication time during the S phase of the cell cycle | [30] | Yes | No | Yes | No | Yes |
Long pause at isochore border | ||||||||
3. | Isochore border between the MHC classes II and class III regions | Replication time during the S phase of the cell cycle | [17] | Yes | Yes | Yes | Yes | Yes |
Long pause at isochore border | ||||||||
4. | Typical isochore length and isochore length distribution subject to isochore GC content | Ultra-centrifugation in combination with fragmentations at different scales. See also theoretical discussions in Constantini et al. | No | Partly | No | Partly | Yes |