Skip to main content
Figure 4 | Genome Biology

Figure 4

From: Modified cell cycle status in a mouse model of altered neuronal vulnerability (slow Wallerian degeneration; Wlds)

Figure 4

Quantitative fluorescent Western blots validate changes in cell cycle proteins in Wlds-expressing HEK293 cells in vitro. Bar chart showing percentage change in protein expression (mean ± standard error of the mean; n ≥ 3 for all proteins) in Wlds-transfected HEK293 cells compared with enhanced green fluorescent protein (eGFP)-transfected control cells. As expected, Wlds protein expression was highly upregulated (left bar). The second portion of the graph shows increases in both pituitary tumor transforming gene 1 (Pttg1) and ubiquitin-activating enzyme E1 (Ube1) proteins following Wldstransfection, both of which were previously implicated in the Wldsneuroprotective phenotype [22, 31]. The third portion of the graph shows validation for two genes highlighted on the SuperArray analysis as being upregulated by more than twofold. The final portion of the graph shows similar increases in cell cycle proteins not included on the SuperArray plate, showing that increased expression of cell cycle proteins is not restricted to those included on the SuperArray. All genes were significantly increased in expression levels in Wlds-transfected cells compared with control cells. **P < 0.01, ***P < 0.001 by unpaired t-test (two-tailed).

Back to article page