Figure 8

Evolutionary sequences of subpopulations in heterogeneous tumors. (a) Frequency histogram (% array probes) of aCGH ratios in a heterogeneous lymphoma is shown, including data for the entire genome. (b) The aCGH ratios are plotted against chromosomal location. The heterogeneous regions on chromosomes 2q, 5p, 7q, 9p, 13q, 20q, and Xp with a DNA copy number of 1&2 and on chromosomes 2p, 4q, 6p, 11q, and 18 with a DNA copy number of 2&3 are shown in blue and red. The blue and red colors represent aberrations that are present in different fractions of the tumor cells; 70% and 30%, respectively. The heterogeneous aberrations are listed in Additional data file 8 except those with a copy number of 2&3, since the lack of 3 DNA copies in this tumor prevented statistical analysis to identify 2&3 heterogeneity. (c) Schematic diagram of two possible evolutionary sequences for the aberrations, one parallel and one serial sequence, are shown. The blue and red circles represent the blue and red aberrations in (b). The percentages indicate the fractions of tumor cells with the listed aberrations, as calculated by GeneCount, showing that the aberrations in blue and red are present in 70% and 30% of the tumor cells, respectively.