Genes differentially regulated in late embryos of ewgl1mutants. (a,b) Functional classification of genes differentially regulated in late embryos of ewgl1mutants. Down-regulated (a) and up-regulated (b) genes were classified according to Gene Ontology processes. (c,d) List of genes differentially regulated in late embryos of ewgl1mutants, giving functional classification and nervous system expression. Hierarchical clustering of normalized expression levels of down-regulated (c) and up-regulated (d) genes in ewgl1embryos compared with wild type and ewgl1embryos rescued with elav-EWG. Gene names and functional categories are shown to the right together with nervous system expression data determined by RNA in situ hybridization (+, expressed in the nervous system; -, not expressed in the nervous system; n.s., no staining; n.d., not determined). Differential expression is visualized by blue (down-regulation) and red (up-regulation). (e) Quantitative RT-PCR of selected genes differentially regulated in ewgl1mutants. PCR products using 32P labeled forward primers from genes up-regulated in ewgl1mutants Ac3, CG7646, CG1909 and Srca (cycles 28, 24, 28 and 28), and from genes down-regulated in ewgl1mutants Pepck, osi14, CG5171 and CG10585 (cycles 26) were analyzed on 6% polyacrylamide gels. elav: control (cycle 28).