Figure 7From: Histone deacetylase inhibition accelerates the early events of stem cell differentiation: transcriptomic and epigenetic analysisEzh2 and H3K27 trimethylation levels on the promoters of Nanog, Sall1 and Nr0b1. (a) ChIP assays using an anti-Ezh2 antibody on the promoters of Nanog, Sall1 and Nr0b1 during TSA treatment (6 and 12 h) and EB formation (4 and 8 days). (b) ChIP assays using an anti-3mK27 antibody were performed for the promoters of TSA down-regulated genes Nanog, Sall1 and Nr0b1 after 6 and 12 h of TSA treatment and further cultivation without TSA for an additional 6 and 12 h.Back to article page