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Table 2 Genes that induce release of dNGLUC activity in SN

From: A pathway sensor for genome-wide screens of intracellular proteolytic cleavage

Gene Fold ratio SN/cells
ASPH 14.2 ± 5.8
BAK1 12.5 ± 2.3
PIR121 12.1 ± 2.7
PERP 10.4 ± 2.5
TBC1D10A 5.5 ± 2.7
FADD 4.9 ± 1.8
BAD 4.8 ± 2.7
CASP8 3.2 ± 1.1
  1. Cells were transfected in 96-well plates with DEVDG2F and cDNA expression vectors from the Origene Trueclone™ collection in triplicates. Activity of GLUC was measured in SN and cell lysates after 26 h and ratios of SN/cellular activity were calculated for each plate. Three wells on each plate were transfected with reporter only to determine the background activity. We show the fold ratio of SN/cellular activity over background averaged from three plates.