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Table 1 dNGLUC is secreted in the absence of a signal peptide

From: A pathway sensor for genome-wide screens of intracellular proteolytic cleavage

 

% secreted

GLUC

96.7 ± 27.6

dNGLUC

30.5 ± 7.8

Actin-dNGLUC

1.5 ± 0.4

  1. GLUC activity was determined in SN and whole cell lysate of 293ET cells transfected with the indicated constructs. The percentage of secreted Gaussia luciferase activity was calculated from three independent transfections.