Figure 5

RT-PCR assays of fusion transcripts on a panel of breast cancer cell lines and normal tissues. HMEC-P1 stands for normal human mammary epithelial cells (passage 1), and HMEC-P4 stands for HMEC passage 4 (higher passage). (a) RT-PCR reveals expression of DR00074 (HYDIN gene fusion) in 16 out of 21 tested breast cancer cell lines, normal cultured human breast epithelial cells, and a wide range of normal human tissues. (b) RT-PCR validation of CN272097 a cDNA produced by a complex rearrangement on chromosome 5 fusing the SLC12A2 gene and expressed sequence tag (EST) AK090949. The results provide evidence for expression of the fused transcript in 5 out of 21 breast cancer cell lines and in higher passage but not lower passage human mammary epithelial cells (HMECs). Note that MDAMB435 was recently demonstrated to be derivative of the M14 melanoma cell line and not from breast [62], and the absence of the SLC12A2 fusion is this cell line is consistent with its absence in other nonbreast tissues.