Subcellular relocalization and signal peptide evolution. Signal peptides (36 amino-terminal residues) and experimentally determined subcellular localizations of the (a) NTG1/NTG2 and (b) TRR1/TRR2 duplicate pairs (derived from the S. cerevisiae WGD event) are shown. K. waltii orthologous sequences are used as outgroups. Predotar [39,40] was used to predict subcellular localizations based on the protein sequences. The (predicted) subcellular localization of the K. waltii proteins was considered to represent the ancestral state. Identical residues in all peptide sequences are represented with (*) under the corresponding position in the protein alignment.