Silencing RELA or NF κ B1 by siRNA significantly altered gene expression. UM-SCC 6 cells were transfected with siRNA to RELA or NF κ B1 for 24 or 48 hours. Total RNA was isolated, and genes selected from NF-κB networks or pathways were analyzed by real time RT-PCR. The data were calculated as the mean plus standard deviation from triplicates with normalization by 18S ribosome RNA, and are presented as the comparison with the cultured cells transfected with the control siRNA oligos. *P < 0.05 (t test).