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Figure 1 | Genome Biology

Figure 1

From: Systems biology-defined NF-κB regulons, interacting signal pathways and networks are implicated in the malignant phenotype of head and neck cancer cell lines differing in p53 status

Figure 1

A schematic diagram of computational, analytic and experimental strategies. COGRIM modeling was performed by integrating four data sources, including microarray analysis of genes differentially expressed by cancer cells, the promoter sequences extracted from genomic databases, NF-κB binding activity in cancer cells, and the NF-κB PWMs from Transfac. The predicted NF-κB target genes were subjected to Ingenuity Pathway Analysis, and NF-κB-associated networks and signaling pathways were identified. The predicted NF-κB target genes were validated by real time RT-PCR, gene knocking down by siRNA, and NF-κB specific binding assays.

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Genome Biology

ISSN: 1474-760X

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