Functionally important segments in proteins dissected using Gene Ontology and geometric clustering of peptide fragments

Table 3 Details of arbitrarily chosen FL8 fragments with propensity ≥20 mapped from GO propensity calculations at level 3

GO MF	Propensity	PDB entry [reference]*	Start^†	Functional description	P-value
0004016	1,816	1azsA [34]	489	VC1 and IIC2 domain interface	0.0006
0019210	1,450	1jsuC [35]	61	Highly conserved β hairpin from p27 interacting with Cdk2 and inhibiting the cyclin-Cdk2 complex	0.0007
0000036	685	1t8kA [33]	19	Part of ligand binding region	0.0014
0016638	450	2bbkL [36]	48	Involved in protein-protein interactions	0.002
0042030	395	1n7lA [32]	13	Important loop connects two helices	0.002
0016566	382	1dvoA [31]	148	Part of large negatively charged region for RNA binding	0.003
0004016	168	1azsA [34]	501	Part of binding pocket of FKP^‡	0.006
0004879	149	1ie9A [37]	288	Forms part of active site pocket	0.007
0016813	137	1wohA [30]	272	One of the active site residues is present	0.007
0016247	107	1oaw [38]	30	Conserved cysteines are present	0.009
0004930	98	1ijyA [29]	113	Surface exposed loop with conserved 'WP' sequence	0.01
0004383	92	1xbnA [39]	74	Forms part of HEM binding pocket	0.01
0005158	61	1qqgB [40]	56	Part of a cationic cluster^§	0.02
0008428	61	1b2uD [41]	39	Interact with the active site residues	0.02
0003724	26	1fukA [42]	341	Conserved interaction with DEAD box motif	0.04

*These proteins do not have a PROSITE sequence signature. The chain identifier is given after the four letter PDB code, wherever present. ^†Residue number as given in PDB. ^‡Only PROSITE domain signature exists: 391-518. ^§Only PROSITE domain signature exists: 12-114.

ISSN: 1474-760X