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Figure 5 | Genome Biology

Figure 5

From: Functions, structure, and read-through alternative splicing of feline APOBEC3 genes

Figure 5

Expression analysis of feline A3C, A3H and A3CH. (a,b) Analysis of expression of feline A3C, A3H and A3CH by RT-PCR of total RNA from feline cell lines (CrFK, MYA-1, KE-R) and feline PBMCs (a) and expression of A3CH in PBMCs of lion, puma, Sumatra-tiger (tiger), and lynx (b). H2O indicates PCRs using primers specific for the A3s without template cDNA added. (c) Analysis of expression of cat A3CH by immunoblot using rabbit serum against the sequence flanked by the C- and H-domains in cat A3C (linker) using 293T cells transfected with A3 expression plasmid or empty vector as indicated and CrFK and MyA-1 cells (two independent cultures each).

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