Figure 3

Validation of siRNA sequences for essential mitotic genes. (a) Protein-protein interaction (PPI) network for candidate genes in which at least one direct interaction (red lines) or indirect interaction (green lines) was identified. The cell cycle phases, based on previous expression analysis, are mapped on each protein bubble based on color. (b) PPI network for SON DNA/RNA-binding protein identified a number of novel components. Proteins in red squares, validated in our follow-up studies, included KIAA1604, FLJ13111/CENP-T, SON, and SMU-1. The green squares represent the nuclear factor-κB proteins examined elsewhere [25].