Figure 2

OPI clustering of high-content screening (HCS) results based on GO/IPR annotations. (a,b) Ontology-based pattern identification (OPI) heat map for each of the morphologic properties recorded in the microscopy based screen. Each row represents a Gene Ontology (GO)/InterPro group consisting of a significant number of annotated genes that shared the representative morphologic profile (P ≤ 0.05). Red and green color represents high and low scores in the corresponding morphological parameters. Headings symbolize morphologic parameters for mitotic index (MI), cell count (CC), nuclear roundness (NR), cell shape (CS), multinucleation (MN), and spindle intensity (SI). Red bars in the PPI and TA columns represent statistically significant support for the gene group based on protein-protein interaction (PPI) and tissue expression (TA) databases; these are determined based on OPI meta-analyses exhibiting multiple protein interactions or mRNA co-expression across 79 tissue samples, respectively. Groups shown in red contain known mitotic or spindle regulation genes, whereas those shown in green contain interesting novel components.