Table 3 Tilled genes and mutation density in Caméor mutant population
From: UTILLdb, a Pisum sativum in silicoforward and reverse genetics tool
Tilled genes | Amplicon size (bp) | % of GC in exons | Identified mutants | Screened M2 families | Mutation frequency |
|---|---|---|---|---|---|
Ps CONSTANS-like a (PsCOLa) | 1,012 | 46.30% | 11 | 1,536 | 1/141 Kb |
LectineA | 971 | 40.80% | 13 | 1,536 | 1/115 Kb |
Sucrose transporter (SUT1) | 1,014 | 52.40% | 12 | 1,536 | 1/130 Kb |
Cell wall invertase (cwINV) | 1,612 | 41.50% | 12 | 1,536 | 1/206 Kb |
Serine-threonine proteine kinase (Sym29) | 2,457 | 44.00% | 8 | 768 | 1/236 Kb |
Phosphoenolpyruvate carboxylase (PepC) | 1,009 | 44.40% | 25 | 3,072 | 1/124 Kb |
Lec1-like (L1L) | 870 | 39.10% | 21 | 4,608 | 1/191 Kb |
DOF transcription factor 2 (PsDOF2) | 1,200 | 36.60% | 9 | 3,072 | 1/410 Kb |
Trypsine inhibitor (TI1) | 712 | 34.20% | 13 | 3,840 | 1/210 Kb |
Pea albumine (PA2) | 746 | 38.50% | 9 | 3,072 | 1/255 Kb |
Anther specific protein (End1) | 851 | 40.50% | 31 | 3,072 | 1/84 Kb |
MADS box gene (PM10) | 1,302 | 34.60% | 20 | 4,608 | 1/300 Kb |
MADS box gene (PM2) | 1,390 | 31.30% | 28 | 4,608 | 1/229 Kb |
Tendril-less transcription factor (TL) | 1,104 | 34.00% | 28 | 3,072 | 1/121 Kb |
Eukaryotic translation initiation factor (eiF4e) | 1,383 | 36.90% | 36 | 4,608 | 1/177 Kb |
Eukaryotic translation initiation factor (eIF(iso)4e) | 772 | 36.70% | 10 | 4,608 | 1/356 Kb |
Methyl transferase 1 (Met1) | 3,842 | 40.20% | 96 | 4,704 | 1/188 Kb |
Retinoblastoma related (RBR) | 2,959 | 40.80% | 72 | 4,608 | 1/112 Kb |
Late embryogenesis abundant protein (PsLEAM) | 952 | 44.00% | 17 | 4,608 | 1/258 Kb |
Heat shock protein 22 (HSP22) | 622 | 45.66% | 18 | 4,608 | 1/159 Kb |
Total/mean | 26,780 | 40.12% | 467 | - | 1/200 Kb |