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Table 1 Effect of EMS

From: UTILLdb, a Pisum sativum in silicoforward and reverse genetics tool

Dose of EMS

0 mM

16 mM

20 mM

24 mM

Total M1 seeds sown

100

1000

4000

3600

Percentage of M1 plants setting seeds

100%

61%

63%

58%

Percentage of M1 plants yielding more than 5 seeds

100%

56%

52%

39%

Percentage of arrested embryos in pods of M1 plants

3%

45%

49%

52%

Mean number of seeds per pod (± SD)

4.83 ± 0.91

2.00 ± 0.86

0.91 ± 1.30

0.79 ± 1.93

  1. Effect of the concentration of EMS on M2 seed setting, on the frequency of arrested embryos (data are expressed as the percentage of total seeds analyzed) and on the mean number of seeds per pod in the M1 generation (200 pods analyzed per dose). SD, standard deviation.