Figure 6
EMSA confirmation experiments. (a) EMSA with established HNF4α recognition sites. Electrophoretic mobility shift experiment with 2.5 μg Caco-2 cell nuclear extracts and oligonucleotides corresponding to promoter regions derived from HNF1, APOB, AAT, AGT, APOC3, CYP2D6, TF, ALDH2, APOC2 and PCK1 as 32P labeled probes. For supershift analysis an antibody directed against HNF4α was added (+). (b) EMSA with predicted novel HNF4α recognition sites. Electrophoretic mobility shift experiment with 2.5 μg Caco-2 cell nuclear extracts and oligonucleotides corresponding to promoter regions derived from NCOA2, TFF2, CHEK1, CD63, SH3GL2, RND2, ESRRBL1, DDB1, NEUROG3 and IL6 as 32P labeled probes. For supershift analysis an antibody directed against HNF4α was added (+). (c) EMSA with potential recognition sites from putative HNF4α targets reported by Odom et al. [11]. Electrophoretic mobility shift experiment with 2.5 μg Caco-2 cell nuclear extracts and oligonucleotides corresponding to promoter regions derived from AZI2, CFL2, GPHN, C14orf119, PPP1R3C, AKR1C3, NPAS2, MDM2, CLCN3 and CBX3 as 32P labeled probes. For supershift analysis an antibody directed against HNF4α was added (+).