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Figure 4 | Genome Biology

Figure 4

From: Temporal regulation of expression of immediate early and second phase transcripts by endothelin-1 in cardiomyocytes

Figure 4

Upregulation of JunB, Egr1, IL-6, c-Fos and Atf3 in cardiomyocytes exposed to ET-1. (a,b,d,e) Cardiomyocytes were exposed to ET-1 for the times indicated. Nuclear extracts were analyzed by western blotting using antibodies to JunB (a), Egr1 (b), c-Fos (d) or Atf3 (e). (c) Cardiomyocytes were unstimulated (Control) or exposed to ET-1 and the tissue culture medium changed and collected at 1 h (0-1 h secretions) then at 2 h (1-2 h secretions). IL-6 in the media was detected by western blotting using slot blotting to transfer proteins to the nitrocellulose. Experiments were repeated with similar results.

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