A genome-wide screen for modifiers of transgene variegation identifies genes with critical roles in development

Table 2 Genotype ratios of offspring at weaning following heterozygous intercrosses

	Observed	Expected if semidominant, homozygous viable	Expected if semidominant, homozygous lethal
MommeD7
Heterozygote*	65		60
Wild type	25		30
MommeD8
Homozygote*	14	27.5
Heterozygote*	56	55
Wild type	40	27.5
MommeD9
Heterozygote*	31		37.3
Wild type	25		18.7
MommeD10
Homozygote* ^†	14	77.25
Heterozygote* ^†	187	154.5
Wild type	108	77.25

Offspring were classified as wild type, heterozygous or homozygous based on their GFP expression profiles. p-values: MommeD7, not significant; MommeD8, p < 0.005; MommeD9, not significant; MommeD10, p < 0.0001. *Progeny testing of mice classified based on GFP expression was carried out in a number of cases: 5 for MommeD7^-/+, 11 for MommeD8^-/-, 16 for MommeD8^-/+, 8 for MommeD9^-/+, 6 for MommeD10^-/- and 11 for MommeD10^-/+. In all cases the genotype was as expected. ^†Presumed genotype was confirmed by genotyping for 8 MommeD10^-/- and 79 MommeD10^-/+ mice.

ISSN: 1474-760X